Pain & CNS behavior CRO
Three-chamber social preference test
This test is commonly used to identify social deficits in mice. The apparatus consist on a large box divided in three chambers with 5-cm openings between chambers. Wired cages are placed on each side chambers. A video-tracking system is used to track mice mouvements.
Three trials are performed for each tested mouse. In the first trial, mice are allowed to freely explore the three chambers of the arena. In the second trial, a wild-type mouse of same age and gender (Stranger1) is placed under one wired cage, the second one is kept empty. In the third trial, a second stranger mouse (Stranger2) is located under the second wired cage.
All the trials are recorded and analyzed post hoc. The time spent in each chamber and time spent in close interaction with stranger mice are measured for each trial. The following parameters are considered:
- social recognition, defined as the ability to identify a conspecific (Stranger1 versus the empty cage) in the second Trial (spending significantly more time in the side-chamber containing Stranger1).
- social novelty/social recognition memory, defined as the ability to discriminate between a novel mouse (Stranger2) and a familiar mouse (Stranger1) in the last trial (spending significantly more time interacting with Stranger2).
Three trials are performed for each tested mouse. In the first trial, mice are allowed to freely explore the three chambers of the arena. In the second trial, a wild-type mouse of same age and gender (Stranger1) is placed under one wired cage, the second one is kept empty. In the third trial, a second stranger mouse (Stranger2) is located under the second wired cage.
All the trials are recorded and analyzed post hoc. The time spent in each chamber and time spent in close interaction with stranger mice are measured for each trial. The following parameters are considered:
- social recognition, defined as the ability to identify a conspecific (Stranger1 versus the empty cage) in the second Trial (spending significantly more time in the side-chamber containing Stranger1).
- social novelty/social recognition memory, defined as the ability to discriminate between a novel mouse (Stranger2) and a familiar mouse (Stranger1) in the last trial (spending significantly more time interacting with Stranger2).
....other tests may also be implemented on demand...
Spared Nerve Injury Model
Chronic Constriction Injury Model
STZ-induced type I Diabetes Neuropathy Model
Carrageenan-induced pain
CFA-induced pain
Formalin-induced pain
Brennan model of incision pain
Phenotype Expertise S.A.R.L. capital social 1.500 euros. SIRET : 794 821 801 00014
