Pain & CNS behavior CRO
Neuropathic Pain Models in Mice
....and rats on demand.
Spared Nerve Injury (SNI) model :
developped by Decorsted & Woolf (2000), the spared nerve injury (SNI) model produces a robust mechanical allodynia measured as a fall in cutaneous sensory threshold. It results from section of the tibial and the common peroneal branches of the sciatic nerve. Neuropathic pain is maintained for several weeks and gives advantage to investigate acquired tolerance of compounds.
Thermal hyperalgesia, mechanical and cold allodynia can be managed.
Chronic Constriction Injury (CCI) model :
developped by Bennett & Xie (1994), this model loosely ties the sciatic nerve with three ligatures at the mid-thigh level. This model gives the advantage to be transient with a recovery of normal state after 3 to 4 weeks. This model is usefull to determine development of neuropathic pain.
Thermal hyperalgeisa, cold allodynia and tactile allodynia are proposed to analyse this model.
STZ-induced type I diabetes neuropathy model :
Diabetes is induced in mice by using streptozotocin (STZ) injection, a compound that has a preferential toxicity toward pancreatic β cells. Neuropathy is a major complication of diabetes.
Readouts of this model are mechanical allodynia and glycemia. Thermal hyperalgesia can also be performed. Pregabalin injection can transiently reversed mechanical allodynia.
developped by Decorsted & Woolf (2000), the spared nerve injury (SNI) model produces a robust mechanical allodynia measured as a fall in cutaneous sensory threshold. It results from section of the tibial and the common peroneal branches of the sciatic nerve. Neuropathic pain is maintained for several weeks and gives advantage to investigate acquired tolerance of compounds.
Thermal hyperalgesia, mechanical and cold allodynia can be managed.
Chronic Constriction Injury (CCI) model :
developped by Bennett & Xie (1994), this model loosely ties the sciatic nerve with three ligatures at the mid-thigh level. This model gives the advantage to be transient with a recovery of normal state after 3 to 4 weeks. This model is usefull to determine development of neuropathic pain.
Thermal hyperalgeisa, cold allodynia and tactile allodynia are proposed to analyse this model.
STZ-induced type I diabetes neuropathy model :
Diabetes is induced in mice by using streptozotocin (STZ) injection, a compound that has a preferential toxicity toward pancreatic β cells. Neuropathy is a major complication of diabetes.
Readouts of this model are mechanical allodynia and glycemia. Thermal hyperalgesia can also be performed. Pregabalin injection can transiently reversed mechanical allodynia.
....other tests may also be implemented on demand....
Spared Nerve Injury Model
Chronic Constriction Injury Model
STZ-induced type I Diabetes Neuropathy Model
Carrageenan-induced pain
CFA-induced pain
Formalin-induced pain
Brennan model of incision pain
Phenotype Expertise S.A.R.L. capital social 1.500 euros. SIRET : 794 821 801 00014
